Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5364139..5364757 Replicon chromosome
Accession NZ_CP032204
Organism Escherichia coli strain AR_0013

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM340_RS27675 Protein ID WP_001291435.1
Coordinates 5364539..5364757 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM340_RS27670 Protein ID WP_000344800.1
Coordinates 5364139..5364513 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM340_RS27660 5359229..5360422 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM340_RS27665 5360445..5363594 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AM340_RS27670 5364139..5364513 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM340_RS27675 5364539..5364757 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM340_RS27680 5364929..5365480 + 552 WP_000536391.1 maltose O-acetyltransferase -
AM340_RS27685 5365596..5366066 + 471 WP_000136192.1 YlaC family protein -
AM340_RS27690 5366230..5367780 + 1551 WP_012602175.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM340_RS27695 5367821..5368174 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AM340_RS27705 5368553..5368864 + 312 WP_000409911.1 MGMT family protein -
AM340_RS27710 5368895..5369467 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111375 WP_001291435.1 NZ_CP032204:5364539-5364757 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T111375 NZ_CP032204:5364539-5364757 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT111375 WP_000344800.1 NZ_CP032204:5364139-5364513 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT111375 NZ_CP032204:5364139-5364513 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References