Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3824197..3824722 Replicon chromosome
Accession NZ_CP032204
Organism Escherichia coli strain AR_0013

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AM340_RS19345 Protein ID WP_001159871.1
Coordinates 3824197..3824502 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AM340_RS19350 Protein ID WP_000813634.1
Coordinates 3824504..3824722 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM340_RS19310 3820217..3820690 + 474 Protein_3574 IS3 family transposase -
AM340_RS19320 3820739..3821131 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
AM340_RS19325 3821136..3822107 - 972 WP_001103694.1 hypothetical protein -
AM340_RS19330 3822336..3822980 + 645 WP_000633911.1 AAA family ATPase -
AM340_RS19335 3822974..3823249 + 276 WP_000239529.1 hypothetical protein -
AM340_RS19340 3823387..3824196 - 810 WP_000016979.1 site-specific integrase -
AM340_RS19345 3824197..3824502 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AM340_RS19350 3824504..3824722 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM340_RS19365 3827044..3827685 + 642 WP_000990668.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - senB 3817207..3853135 35928


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T111364 WP_001159871.1 NZ_CP032204:c3824502-3824197 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T111364 NZ_CP032204:c3824502-3824197 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT111364 WP_000813634.1 NZ_CP032204:c3824722-3824504 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT111364 NZ_CP032204:c3824722-3824504 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References