Detailed information of TA system TA111359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3391390..3392174
• Replicon: chromosome
• Accession: NZ_CP032204
• Organism: Escherichia coli strain AR_0013

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: AM340_RS16865
• Protein ID: WP_000613629.1
• Coordinates: 3391390..3391884 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: AM340_RS16870
• Protein ID: WP_001110446.1
• Coordinates: 3391881..3392174 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM340_RS16845	3386583..3387680	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
AM340_RS16850	3387680..3388621	+	942	WP_012602385.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
AM340_RS16855	3388687..3390330	+	1644	WP_000096449.1	flagellar hook-associated protein FlgK	-
AM340_RS16860	3390342..3391295	+	954	WP_001212748.1	flagellar hook-associated protein FlgL	-
AM340_RS16865	3391390..3391884	-	495	WP_000613629.1	GNAT family N-acetyltransferase	Toxin
AM340_RS16870	3391881..3392174	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
AM340_RS16875	3392308..3395493	-	3186	WP_119164920.1	ribonuclease E	-
AM340_RS16885	3396066..3397025	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18112.05 Da
• Isoelectric Point: 8.2617

>T111359 WP_000613629.1 NZ_CP032204:c3391884-3391390 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRVLKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T111359 NZ_CP032204:c3391884-3391390 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGTCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT111359 WP_001110446.1 NZ_CP032204:c3392174-3391881 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT111359 NZ_CP032204:c3392174-3391881 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775