Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2465546..2466129 Replicon chromosome
Accession NZ_CP032204
Organism Escherichia coli strain AR_0013

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag AM340_RS12395 Protein ID WP_000254738.1
Coordinates 2465794..2466129 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1M2IZI3
Locus tag AM340_RS12390 Protein ID WP_000581940.1
Coordinates 2465546..2465794 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM340_RS12380 2461885..2463186 + 1302 WP_000046807.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
AM340_RS12385 2463234..2465468 + 2235 WP_000226815.1 GTP pyrophosphokinase -
AM340_RS12390 2465546..2465794 + 249 WP_000581940.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
AM340_RS12395 2465794..2466129 + 336 WP_000254738.1 endoribonuclease MazF Toxin
AM340_RS12400 2466201..2466992 + 792 WP_001071660.1 nucleoside triphosphate pyrophosphohydrolase -
AM340_RS12405 2467220..2468857 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
AM340_RS12410 2468944..2470242 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T111358 WP_000254738.1 NZ_CP032204:2465794-2466129 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T111358 NZ_CP032204:2465794-2466129 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTCAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTTGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9270.67 Da        Isoelectric Point: 4.5972

>AT111358 WP_000581940.1 NZ_CP032204:2465546-2465794 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIGLVDGKLIIEPVRKEPVFTLAELVSDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT111358 NZ_CP032204:2465546-2465794 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCAGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGGCCTGGTGGATGGCAAATTAATTATTGAGCCAGTACGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAGCGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IZI3

References