Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2336528..2337182 Replicon chromosome
Accession NZ_CP032204
Organism Escherichia coli strain AR_0013

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AM340_RS11855 Protein ID WP_000244781.1
Coordinates 2336775..2337182 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AM340_RS11850 Protein ID WP_000354046.1
Coordinates 2336528..2336794 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM340_RS11830 2332616..2334049 - 1434 WP_001530823.1 6-phospho-beta-glucosidase BglA -
AM340_RS11835 2334094..2334405 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AM340_RS11840 2334569..2335228 + 660 WP_000250274.1 hemolysin III family protein -
AM340_RS11845 2335305..2336285 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
AM340_RS11850 2336528..2336794 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AM340_RS11855 2336775..2337182 + 408 WP_000244781.1 protein YgfX Toxin
AM340_RS11860 2337222..2337743 - 522 WP_001055874.1 flavodoxin FldB -
AM340_RS11865 2337855..2338751 + 897 WP_021521522.1 site-specific tyrosine recombinase XerD -
AM340_RS11870 2338776..2339486 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AM340_RS11875 2339492..2341225 + 1734 WP_000813239.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T111357 WP_000244781.1 NZ_CP032204:2336775-2337182 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T111357 NZ_CP032204:2336775-2337182 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT111357 WP_000354046.1 NZ_CP032204:2336528-2336794 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT111357 NZ_CP032204:2336528-2336794 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References