Detailed information of TA system TA111353

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1379765..1380599
• Replicon: chromosome
• Accession: NZ_CP032204
• Organism: Escherichia coli strain AR_0013

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8T5WFL4
• Locus tag: AM340_RS06880
• Protein ID: WP_000854823.1
• Coordinates: 1379765..1380142 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8T5WFE1
• Locus tag: AM340_RS06885
• Protein ID: WP_001285614.1
• Coordinates: 1380231..1380599 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM340_RS06845	1375406..1376329	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
AM340_RS06850	1376440..1377624	-	1185	WP_001172866.1	sugar efflux transporter	-
AM340_RS06855	1378053..1378181	-	129	Protein_1252	virulence RhuM family protein	-
AM340_RS06865	1378419..1379261	-	843	WP_001290170.1	DUF4942 domain-containing protein	-
AM340_RS06870	1379346..1379543	-	198	WP_087890155.1	DUF957 domain-containing protein	-
AM340_RS06875	1379619..1379768	-	150	Protein_1255	hypothetical protein	-
AM340_RS06880	1379765..1380142	-	378	WP_000854823.1	TA system toxin CbtA family protein	Toxin
AM340_RS06885	1380231..1380599	-	369	WP_001285614.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM340_RS06890	1380679..1380900	-	222	WP_000692350.1	DUF987 domain-containing protein	-
AM340_RS06895	1380987..1381463	-	477	WP_001186792.1	RadC family protein	-
AM340_RS06900	1381479..1381958	-	480	WP_000706978.1	antirestriction protein	-
AM340_RS06905	1382224..1383042	-	819	WP_001175143.1	DUF945 domain-containing protein	-
AM340_RS06910	1383132..1383365	-	234	WP_000883181.1	DUF905 family protein	-
AM340_RS06915	1383371..1384048	-	678	WP_001097562.1	hypothetical protein	-
AM340_RS06920	1384199..1384879	-	681	WP_001278651.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1368698..1393756	25058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.04 Da
• Isoelectric Point: 6.7093

>T111353 WP_000854823.1 NZ_CP032204:c1380142-1379765 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T111353 NZ_CP032204:c1380142-1379765 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATGACATTAGCCAGGGGAAGTATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13596.35 Da
• Isoelectric Point: 6.0618

>AT111353 WP_001285614.1 NZ_CP032204:c1380599-1380231 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTSCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT111353 NZ_CP032204:c1380599-1380231 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GTCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA