Detailed information of TA system TA111345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 106806..107331
• Replicon: plasmid unnamed1
• Accession: NZ_CP032202
• Organism: Escherichia coli strain AR_0086

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: AM465_RS27205
• Protein ID: WP_001159868.1
• Coordinates: 107026..107331 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: AM465_RS27200
• Protein ID: WP_000813634.1
• Coordinates: 106806..107024 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM465_RS27155	101806..102165	-	360	WP_001513660.1	hypothetical protein	-
AM465_RS27160	102193..102372	-	180	WP_001513661.1	hypothetical protein	-
AM465_RS27165	102377..102757	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	-
AM465_RS27170	102757..102978	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AM465_RS27175	103161..103277	+	117	Protein_122	type I restriction endonuclease subunit M	-
AM465_RS27185	103333..104030	+	698	Protein_123	IS1 family transposase	-
AM465_RS27190	104167..105099	-	933	WP_000991832.1	hypothetical protein	-
AM465_RS27195	105103..106098	-	996	WP_000246636.1	hypothetical protein	-
AM465_RS27200	106806..107024	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AM465_RS27205	107026..107331	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
AM465_RS27210	107332..108138	+	807	WP_000016982.1	site-specific integrase	-
AM465_RS27215	108912..109667	+	756	WP_000852146.1	replication initiation protein RepE	-
AM465_RS27220	110255..111421	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..140509	140509
	-	inside	IScluster/Tn	-	-	96597..104030	7433

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T111345 WP_001159868.1 NZ_CP032202:107026-107331 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T111345 NZ_CP032202:107026-107331 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT111345 WP_000813634.1 NZ_CP032202:106806-107024 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT111345 NZ_CP032202:106806-107024 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2