Detailed information of TA system TA111316

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 183508..184343
• Replicon: chromosome
• Accession: NZ_CP032201
• Organism: Escherichia coli strain AR_0086

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: AM465_RS01055
• Protein ID: WP_000854759.1
• Coordinates: 183508..183885 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: AM465_RS01060
• Protein ID: WP_001295723.1
• Coordinates: 183975..184343 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM465_RS01030	179619..181259	-	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
AM465_RS01035	182032..182208	-	177	Protein_184	helix-turn-helix domain-containing protein	-
AM465_RS27755	182572..182730	-	159	WP_001467148.1	hypothetical protein	-
AM465_RS01045	182830..183006	-	177	WP_000839286.1	DUF957 domain-containing protein	-
AM465_RS01050	183023..183511	-	489	WP_000761690.1	hypothetical protein	-
AM465_RS01055	183508..183885	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
AM465_RS01060	183975..184343	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM465_RS01065	184506..184727	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM465_RS01070	184790..185266	-	477	WP_059333688.1	RadC family protein	-
AM465_RS01075	185282..185755	-	474	WP_001542276.1	antirestriction protein	-
AM465_RS01085	186096..186833	-	738	Protein_193	DUF932 domain-containing protein	-
AM465_RS01090	186919..187665	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
AM465_RS01095	187680..189221	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T111316 WP_000854759.1 NZ_CP032201:c183885-183508 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T111316 NZ_CP032201:c183885-183508 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT111316 WP_001295723.1 NZ_CP032201:c184343-183975 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT111316 NZ_CP032201:c184343-183975 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52