Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 582554..583173 Replicon chromosome
Accession NZ_CP032200
Organism Klebsiella pneumoniae strain AR_0097

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM476_RS05260 Protein ID WP_002892050.1
Coordinates 582955..583173 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AM476_RS05255 Protein ID WP_002892066.1
Coordinates 582554..582928 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM476_RS05245 577706..578899 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM476_RS05250 578922..582068 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AM476_RS05255 582554..582928 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AM476_RS05260 582955..583173 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM476_RS05265 583332..583898 + 567 WP_002892030.1 maltose O-acetyltransferase -
AM476_RS29475 583870..584010 - 141 WP_004147370.1 hypothetical protein -
AM476_RS05270 584031..584501 + 471 WP_002892026.1 YlaC family protein -
AM476_RS05275 584476..585927 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
AM476_RS05280 586028..586726 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
AM476_RS05285 586723..586863 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AM476_RS05290 586863..587126 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111296 WP_002892050.1 NZ_CP032200:582955-583173 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T111296 NZ_CP032200:582955-583173 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT111296 WP_002892066.1 NZ_CP032200:582554-582928 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT111296 NZ_CP032200:582554-582928 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References