Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41833..42476 Replicon plasmid unnamed3
Accession NZ_CP032197
Organism Klebsiella pneumoniae strain AR_0097

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM476_RS01785 Protein ID WP_001044770.1
Coordinates 41833..42249 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM476_RS01790 Protein ID WP_001261282.1
Coordinates 42246..42476 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM476_RS29280 37724..37789 + 66 Protein_41 EAL domain-containing protein -
AM476_RS01750 37929..38693 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
AM476_RS01760 38836..39102 - 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
AM476_RS01765 39323..39796 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
AM476_RS01770 39952..40719 + 768 Protein_45 class 1 integron integrase IntI1 -
AM476_RS01775 40772..41476 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM476_RS01780 41523..41759 - 237 WP_001348075.1 AAA family ATPase -
AM476_RS01785 41833..42249 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM476_RS01790 42246..42476 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM476_RS01795 42433..42893 + 461 Protein_50 hypothetical protein -
AM476_RS29285 43021..43341 + 321 WP_162896398.1 hypothetical protein -
AM476_RS01800 43390..44028 + 639 WP_022644887.1 hypothetical protein -
AM476_RS01805 44031..44816 + 786 WP_022644886.1 site-specific integrase -
AM476_RS01810 44874..45131 - 258 WP_032408936.1 hypothetical protein -
AM476_RS29290 45260..45373 - 114 WP_032072094.1 hypothetical protein -
AM476_RS01820 46004..46759 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..73502 73502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T111294 WP_001044770.1 NZ_CP032197:c42249-41833 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T111294 NZ_CP032197:c42249-41833 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT111294 WP_001261282.1 NZ_CP032197:c42476-42246 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT111294 NZ_CP032197:c42476-42246 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References