Detailed information of TA system TA111292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54334..54857
• Replicon: plasmid unnamed3
• Accession: NZ_CP032188
• Organism: Klebsiella pneumoniae strain AR_0075

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V5RNQ6
• Locus tag: AM454_RS28955
• Protein ID: WP_015632464.1
• Coordinates: 54552..54857 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V5RQR8
• Locus tag: AM454_RS28950
• Protein ID: WP_015632465.1
• Coordinates: 54334..54552 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM454_RS28930	50477..51748	-	1272	WP_016162068.1	Y-family DNA polymerase	-
AM454_RS28935	51748..52179	-	432	WP_015632467.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
AM454_RS28940	52343..52828	-	486	Protein_62	IS5 family transposase	-
AM454_RS28945	53123..54148	-	1026	WP_015632466.1	IS110 family transposase	-
AM454_RS28950	54334..54552	+	219	WP_015632465.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AM454_RS28955	54552..54857	+	306	WP_015632464.1	type II toxin-antitoxin system toxin CcdB	Toxin
AM454_RS28960	55002..55346	+	345	WP_015632463.1	hypothetical protein	-
AM454_RS28965	55388..55954	+	567	WP_015632462.1	hypothetical protein	-
AM454_RS28970	56158..56415	-	258	WP_016162071.1	helix-turn-helix domain-containing protein	-
AM454_RS28975	56845..57072	+	228	WP_016162072.1	hypothetical protein	-
AM454_RS28980	57086..57208	+	123	Protein_70	transcriptional regulator	-
AM454_RS28985	57414..57857	-	444	WP_015632459.1	type II toxin-antitoxin system HicB family antitoxin	-
AM454_RS28990	57883..58065	-	183	WP_015632458.1	type II toxin-antitoxin system HicA family toxin	-
AM454_RS28995	58426..59406	+	981	WP_015632457.1	partitioning protein	-
AM454_RS29000	59399..59827	+	429	WP_015632456.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ARR-3 / rmtF / blaCTX-M-15	-	1..93870	93870
	-	flank	IS/Tn	-	-	53123..54148	1025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11361.90 Da
• Isoelectric Point: 5.5252

>T111292 WP_015632464.1 NZ_CP032188:54552-54857 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Nucleotide

• Length: 306 bp

>T111292 NZ_CP032188:54552-54857 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8194.13 Da
• Isoelectric Point: 6.2224

>AT111292 WP_015632465.1 NZ_CP032188:54334-54552 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Nucleotide

• Length: 219 bp

>AT111292 NZ_CP032188:54334-54552 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Structures

Toxin

• AlphaFold DB: A0A6C0ND19

Antitoxin

• AlphaFold DB: A0A6C0L1T7