Detailed information of TA system TA111291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 43219..43862
• Replicon: plasmid unnamed3
• Accession: NZ_CP032188
• Organism: Klebsiella pneumoniae strain AR_0075

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AM454_RS28880
• Protein ID: WP_071854281.1
• Coordinates: 43219..43635 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AM454_RS28885
• Protein ID: WP_001261282.1
• Coordinates: 43632..43862 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM454_RS28860	40671..41003	+	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-
AM454_RS28865	41050..41925	-	876	WP_000239590.1	class A extended-spectrum beta-lactamase CTX-M-15	-
AM454_RS28870	42158..42862	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
AM454_RS28875	42909..43145	-	237	WP_001348075.1	AAA family ATPase	-
AM454_RS28880	43219..43635	-	417	WP_071854281.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM454_RS28885	43632..43862	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM454_RS28890	43819..44280	+	462	WP_162899471.1	hypothetical protein	-
AM454_RS28895	44425..44739	+	315	WP_015632378.1	hypothetical protein	-
AM454_RS28900	44788..45099	+	312	WP_024191724.1	hypothetical protein	-
AM454_RS28905	45164..46168	+	1005	WP_016162067.1	hypothetical protein	-
AM454_RS31870	46212..46373	+	162	WP_032495756.1	hypothetical protein	-
AM454_RS28910	46366..47160	+	795	WP_015632375.1	site-specific integrase	-
AM454_RS28915	47632..47811	-	180	WP_006788217.1	hypothetical protein	-
AM454_RS28920	47931..48557	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ARR-3 / rmtF / blaCTX-M-15	-	1..93870	93870

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15086.51 Da
• Isoelectric Point: 7.1084

>T111291 WP_071854281.1 NZ_CP032188:c43635-43219 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T111291 NZ_CP032188:c43635-43219 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTTGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT111291 WP_001261282.1 NZ_CP032188:c43862-43632 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT111291 NZ_CP032188:c43862-43632 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3