Detailed information of TA system TA111290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 198558..199303
• Replicon: plasmid unnamed1
• Accession: NZ_CP032186
• Organism: Klebsiella pneumoniae strain AR_0075

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: AM454_RS27975
• Protein ID: WP_032408901.1
• Coordinates: 198558..199049 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: AM454_RS27980
• Protein ID: WP_014386183.1
• Coordinates: 199037..199303 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM454_RS27950	194242..194997	+	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-
AM454_RS27955	195259..195699	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
AM454_RS27960	195696..196046	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
AM454_RS27965	196077..197669	+	1593	Protein_211	IS66 family transposase	-
AM454_RS27970	197866..198117	+	252	WP_032408900.1	aldo/keto reductase	-
AM454_RS27975	198558..199049	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
AM454_RS27980	199037..199303	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
AM454_RS27985	199500..199658	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
AM454_RS27990	199902..200387	-	486	WP_014386185.1	hypothetical protein	-
AM454_RS27995	200577..200849	+	273	WP_032408902.1	hypothetical protein	-
AM454_RS28000	200846..201196	+	351	WP_014386186.1	hypothetical protein	-
AM454_RS28015	201833..202159	+	327	WP_014386187.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / tet(A)	-	1..202373	202373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T111290 WP_032408901.1 NZ_CP032186:c199049-198558 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T111290 NZ_CP032186:c199049-198558 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT111290 WP_014386183.1 NZ_CP032186:c199303-199037 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT111290 NZ_CP032186:c199303-199037 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6