Detailed information of TA system TA111289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 174764..175407
• Replicon: plasmid unnamed1
• Accession: NZ_CP032186
• Organism: Klebsiella pneumoniae strain AR_0075

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AM454_RS27830
• Protein ID: WP_014386165.1
• Coordinates: 174764..175180 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: AM454_RS27835
• Protein ID: WP_032408893.1
• Coordinates: 175177..175407 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM454_RS27805	171384..171815	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
AM454_RS27810	171812..172540	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
AM454_RS27815	172537..172863	+	327	WP_004152639.1	hypothetical protein	-
AM454_RS27820	172919..173293	+	375	WP_032408891.1	hypothetical protein	-
AM454_RS27825	173473..174441	-	969	WP_074422984.1	IS5 family transposase	-
AM454_RS27830	174764..175180	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
AM454_RS27835	175177..175407	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM454_RS27845	175727..176161	+	435	WP_000103648.1	RidA family protein	-
AM454_RS27850	176166..176840	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
AM454_RS27855	176856..178013	+	1158	WP_032408895.1	acetylornithine deacetylase	-
AM454_RS27860	178184..179332	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
AM454_RS27865	179339..180334	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / tet(A)	-	1..202373	202373
	-	flank	IS/Tn	-	-	173473..174441	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T111289 WP_014386165.1 NZ_CP032186:c175180-174764 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T111289 NZ_CP032186:c175180-174764 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT111289 WP_032408893.1 NZ_CP032186:c175407-175177 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT111289 NZ_CP032186:c175407-175177 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA