Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 100145..100881 Replicon plasmid unnamed1
Accession NZ_CP032186
Organism Klebsiella pneumoniae strain AR_0075

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM454_RS27390 Protein ID WP_003026803.1
Coordinates 100145..100627 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM454_RS27395 Protein ID WP_003026799.1
Coordinates 100615..100881 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM454_RS27375 95782..96744 + 963 WP_004152113.1 M48 family metalloprotease -
AM454_RS27380 96832..98086 + 1255 Protein_107 IS3 family transposase -
AM454_RS27385 98587..99933 - 1347 WP_020314316.1 ISNCY family transposase -
AM454_RS27390 100145..100627 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM454_RS27395 100615..100881 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM454_RS27400 101057..101311 - 255 WP_004152108.1 hypothetical protein -
AM454_RS27405 101387..101644 - 258 WP_004152107.1 hypothetical protein -
AM454_RS27410 101693..101896 - 204 WP_004152106.1 hemolysin expression modulator Hha -
AM454_RS27415 101930..102298 - 369 WP_004152105.1 hypothetical protein -
AM454_RS27420 102342..102836 - 495 WP_004152104.1 DNA-binding protein -
AM454_RS27425 102867..103442 - 576 WP_004152103.1 hypothetical protein -
AM454_RS27430 103430..103699 - 270 WP_004152102.1 hypothetical protein -
AM454_RS27435 104057..104407 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM454_RS27440 104457..104819 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / tet(A) - 1..202373 202373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T111288 WP_003026803.1 NZ_CP032186:c100627-100145 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T111288 NZ_CP032186:c100627-100145 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT111288 WP_003026799.1 NZ_CP032186:c100881-100615 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT111288 NZ_CP032186:c100881-100615 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References