Detailed information of TA system TA111286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4848575..4849200
• Replicon: chromosome
• Accession: NZ_CP032185
• Organism: Klebsiella pneumoniae strain AR_0075

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM454_RS24510
• Protein ID: WP_040182550.1
• Coordinates: 4848817..4849200 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: AM454_RS24505
• Protein ID: WP_004150355.1
• Coordinates: 4848575..4848817 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM454_RS24480	4844566..4845165	+	600	WP_004151865.1	glucose-1-phosphatase	-
AM454_RS24485	4845159..4846019	+	861	WP_004146232.1	virulence factor BrkB family protein	-
AM454_RS24490	4846016..4846453	+	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM454_RS24495	4846498..4847439	+	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
AM454_RS24500	4847453..4848370	-	918	WP_023302328.1	alpha/beta hydrolase	-
AM454_RS24505	4848575..4848817	+	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM454_RS24510	4848817..4849200	+	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM454_RS24515	4849374..4850303	-	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
AM454_RS24520	4850300..4850935	-	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
AM454_RS24525	4850932..4851834	-	903	WP_002882822.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T111286 WP_040182550.1 NZ_CP032185:4848817-4849200 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T111286 NZ_CP032185:4848817-4849200 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGAAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT111286 WP_004150355.1 NZ_CP032185:4848575-4848817 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT111286 NZ_CP032185:4848575-4848817 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9