Detailed information of TA system TA111270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 5406367..5406957
• Replicon: chromosome
• Accession: NZ_CP032178
• Organism: Klebsiella pneumoniae strain AR_0135

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AM382_RS27900
• Protein ID: WP_023341911.1
• Coordinates: 5406367..5406699 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: AM382_RS27905
• Protein ID: WP_000288812.1
• Coordinates: 5406700..5406957 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM382_RS27875	5402414..5402659	+	246	WP_004141189.1	signal transduction protein PmrD	-
AM382_RS27880	5402789..5404243	+	1455	WP_004148975.1	AMP nucleosidase	-
AM382_RS27890	5404609..5406045	-	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
AM382_RS27900	5406367..5406699	-	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AM382_RS27905	5406700..5406957	-	258	WP_000288812.1	antitoxin	Antitoxin
AM382_RS27915	5407292..5407498	-	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
AM382_RS27920	5407495..5407956	-	462	WP_004213450.1	hypothetical protein	-
AM382_RS27925	5408239..5408820	-	582	WP_099184326.1	hypothetical protein	-
AM382_RS27930	5408874..5410622	-	1749	WP_032418698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T111270 WP_023341911.1 NZ_CP032178:c5406699-5406367 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T111270 NZ_CP032178:c5406699-5406367 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT111270 WP_000288812.1 NZ_CP032178:c5406957-5406700 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT111270 NZ_CP032178:c5406957-5406700 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0