Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 51684..52327 Replicon plasmid unnamed1
Accession NZ_CP032176
Organism Klebsiella pneumoniae strain AR_0135

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM382_RS00380 Protein ID WP_001044770.1
Coordinates 51911..52327 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM382_RS00375 Protein ID WP_001261282.1
Coordinates 51684..51914 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM382_RS00345 47256..48122 - 867 WP_004118283.1 replication initiation protein -
AM382_RS28030 48657..48761 + 105 WP_032409716.1 hypothetical protein -
AM382_RS00350 48890..49147 + 258 WP_000764642.1 hypothetical protein -
AM382_RS00355 49205..49981 - 777 WP_000015958.1 site-specific integrase -
AM382_RS00360 49978..50721 - 744 WP_000129823.1 hypothetical protein -
AM382_RS00365 50772..51122 - 351 WP_000493378.1 hypothetical protein -
AM382_RS00370 51266..51727 - 462 WP_074192227.1 hypothetical protein -
AM382_RS00375 51684..51914 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM382_RS00380 51911..52327 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM382_RS00385 52401..53111 + 711 WP_004152334.1 ATP-binding protein -
AM382_RS00390 53145..53842 + 698 Protein_63 IS1 family transposase -
AM382_RS00395 53858..53968 + 111 Protein_64 mercuric transport protein periplasmic component -
AM382_RS00400 54004..54426 + 423 WP_001340589.1 organomercurial transporter MerC -
AM382_RS00405 54478..56172 + 1695 WP_000105636.1 mercury(II) reductase -
AM382_RS00410 56190..56552 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
AM382_RS00415 56549..56785 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA14 / tet(D) / sul1 / qacE / catB2 / ant(3'')-Ia / blaVIM-1 / blaSHV-12 / aac(6')-Ib / blaOXA-9 / blaTEM-1A / aac(3)-IIa - 1..90682 90682


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T111253 WP_001044770.1 NZ_CP032176:51911-52327 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T111253 NZ_CP032176:51911-52327 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT111253 WP_001261282.1 NZ_CP032176:51684-51914 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT111253 NZ_CP032176:51684-51914 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References