Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3359691..3360310 Replicon chromosome
Accession NZ_CP032175
Organism Klebsiella pneumoniae strain AR_0160

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM406_RS17930 Protein ID WP_002892050.1
Coordinates 3359691..3359909 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AM406_RS17935 Protein ID WP_002892066.1
Coordinates 3359936..3360310 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM406_RS17900 3355738..3356001 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
AM406_RS17905 3356001..3356141 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AM406_RS17910 3356138..3356836 - 699 WP_004177238.1 GNAT family N-acetyltransferase -
AM406_RS17915 3356937..3358388 + 1452 WP_004177237.1 PLP-dependent aminotransferase family protein -
AM406_RS17920 3358363..3358833 - 471 WP_002892026.1 YlaC family protein -
AM406_RS28360 3358854..3358994 + 141 WP_004147370.1 hypothetical protein -
AM406_RS17925 3358966..3359532 - 567 WP_002892030.1 maltose O-acetyltransferase -
AM406_RS17930 3359691..3359909 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM406_RS17935 3359936..3360310 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AM406_RS17940 3360796..3363942 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AM406_RS17945 3363965..3365158 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111251 WP_002892050.1 NZ_CP032175:c3359909-3359691 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T111251 NZ_CP032175:c3359909-3359691 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT111251 WP_002892066.1 NZ_CP032175:c3360310-3359936 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT111251 NZ_CP032175:c3360310-3359936 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References