Detailed information of TA system TA111250

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 3163460..3164177
• Replicon: chromosome
• Accession: NZ_CP032175
• Organism: Klebsiella pneumoniae strain AR_0160

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: AM406_RS16945
• Protein ID: WP_071994601.1
• Coordinates: 3163460..3163792 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8B4G4X8
• Locus tag: AM406_RS16950
• Protein ID: WP_032410127.1
• Coordinates: 3163827..3164177 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM406_RS16930	3158820..3159779	-	960	WP_002889884.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-
AM406_RS16935	3160153..3162798	+	2646	WP_025983219.1	LuxR family transcriptional regulator	-
AM406_RS16945	3163460..3163792	-	333	WP_071994601.1	TA system toxin CbtA family protein	Toxin
AM406_RS16950	3163827..3164177	-	351	WP_032410127.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM406_RS16955	3164198..3164419	-	222	WP_004129348.1	DUF987 domain-containing protein	-
AM406_RS16960	3164435..3164908	-	474	WP_032410128.1	DNA repair protein RadC	-
AM406_RS16965	3164979..3165800	-	822	WP_162897387.1	DUF945 domain-containing protein	-
AM406_RS16970	3166078..3166842	-	765	WP_004184534.1	IS21-like element helper ATPase IstB	-
AM406_RS16975	3166839..3168338	-	1500	WP_004184532.1	IS21 family transposase	-
AM406_RS16980	3168431..3168988	-	558	Protein_2948	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (15-115)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12513.46 Da
• Isoelectric Point: 7.2132

>T111250 WP_071994601.1 NZ_CP032175:c3163792-3163460 [Klebsiella pneumoniae]
MNTLPAINQRAVQTCLSPVVVWQMLLARLLEQHYGLTLNDTPFSDEAVLKRHIEAGITLVDAVNFLVEKYELIRIDRRGF
SSQGQVPYLTVTDILHARRECGLTNSCPYR

Nucleotide

• Length: 333 bp

>T111250 NZ_CP032175:c3163792-3163460 [Klebsiella pneumoniae]
ATGAATACTTTACCTGCAATAAACCAGCGGGCTGTTCAGACATGCTTATCACCCGTGGTTGTCTGGCAAATGCTGCTTGC
TCGACTACTTGAGCAGCACTATGGCCTGACCTTAAATGACACGCCGTTCAGCGATGAGGCTGTTCTTAAAAGACATATCG
AAGCTGGTATCACGCTTGTCGATGCCGTTAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGATCGCAGAGGATTT
AGCTCACAGGGACAAGTGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGAATGTGGATTAACGAACAGTTG
TCCTTATCGGTAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13014.93 Da
• Isoelectric Point: 7.1865

>AT111250 WP_032410127.1 NZ_CP032175:c3164177-3163827 [Klebsiella pneumoniae]
MSSKTLTVNDDTAEPWWGLNRNVIPCFGARLVQEGNRLYYLSDRASIIGLFNKADLLRLDQAFPVLLKQAELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSCGHVYISIYPTQR

Nucleotide

• Length: 351 bp

>AT111250 NZ_CP032175:c3164177-3163827 [Klebsiella pneumoniae]
ATGTCGAGTAAGACACTCACAGTGAATGATGATACCGCTGAGCCCTGGTGGGGGCTGAATCGTAATGTCATTCCTTGTTT
TGGTGCGCGTCTGGTCCAGGAAGGTAACCGCCTATATTACCTGTCTGACCGCGCCAGCATAATAGGCCTATTCAATAAAG
CGGATTTGCTCCGTCTTGACCAGGCATTTCCTGTTCTGCTGAAGCAGGCGGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACCCTCTATGCAAAGGGGCTGACCTGCGAAGCTGATACACTGGGTTCCTGTGGTCACGT
CTATATCTCCATTTATCCTACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B4G4X8