Detailed information of TA system TA111248

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 2700219..2700922
• Replicon: chromosome
• Accession: NZ_CP032175
• Organism: Klebsiella pneumoniae strain AR_0160

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: AM406_RS14670
• Protein ID: WP_127717242.1
• Coordinates: 2700626..2700922 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2Z3WZC0
• Locus tag: AM406_RS14665
• Protein ID: WP_032410777.1
• Coordinates: 2700219..2700560 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM406_RS14630	2695370..2696245	+	876	WP_032410762.1	GTPase family protein	-
AM406_RS14635	2696489..2697205	+	717	WP_053064560.1	WYL domain-containing protein	-
AM406_RS14640	2697241..2697693	+	453	WP_032410767.1	hypothetical protein	-
AM406_RS14645	2697765..2698238	+	474	WP_032410769.1	hypothetical protein	-
AM406_RS14650	2698358..2699179	+	822	WP_032410772.1	DUF945 domain-containing protein	-
AM406_RS14655	2699210..2699653	+	444	WP_032410774.1	antirestriction protein	-
AM406_RS14660	2699666..2700208	+	543	WP_032410776.1	DNA repair protein RadC	-
AM406_RS14665	2700219..2700560	+	342	WP_032410777.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM406_RS14670	2700626..2700922	+	297	WP_127717242.1	TA system toxin CbtA family protein	Toxin
AM406_RS14675	2701186..2702193	+	1008	WP_032410782.1	restriction endonuclease	-
AM406_RS14685	2702561..2703672	+	1112	Protein_2522	IS3 family transposase	-
AM406_RS14690	2703691..2704014	+	324	Protein_2523	hypothetical protein	-
AM406_RS14695	2704206..2704685	-	480	WP_002887049.1	type VI secretion system tube protein Hcp	-
AM406_RS14700	2704688..2705035	-	348	WP_004199257.1	hypothetical protein	-
AM406_RS14705	2705037..2705711	-	675	WP_004152211.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2681536..2705711	24175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-94)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11195.82 Da
• Isoelectric Point: 7.2019

>T111248 WP_127717242.1 NZ_CP032175:2700626-2700922 [Klebsiella pneumoniae]
MSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRVDRRGFDWQEQSPYLRAVDIL
RARQATGLLKRNRISAAR

Nucleotide

• Length: 297 bp

>T111248 NZ_CP032175:2700626-2700922 [Klebsiella pneumoniae]
CTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGCACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCC
ATTCAGTGATGAAACGGTAATTCAGGAACATATCGATGCCGGTATATCTTTGGCCGATGCTGTTAATTTTCTGGTGGAAA
AGTACGGGCTGGTTCGTGTTGATCGCAGGGGATTCGACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTG
CGGGCGCGACAGGCAACAGGTTTATTGAAAAGAAATCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12765.40 Da
• Isoelectric Point: 7.0269

>AT111248 WP_032410777.1 NZ_CP032175:2700219-2700560 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHRVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT111248 NZ_CP032175:2700219-2700560 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCGCATTTTATCAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACCGCGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z3WZC0