Detailed information of TA system TA111233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 24596..25118
• Replicon: plasmid unnamed1
• Accession: NZ_CP032173
• Organism: Klebsiella pneumoniae strain AR_0160

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: AM406_RS00150
• Protein ID: WP_004187019.1
• Coordinates: 24596..24880 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: AM406_RS00155
• Protein ID: WP_004187025.1
• Coordinates: 24870..25118 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM406_RS00125	19815..20279	-	465	WP_004187010.1	hypothetical protein	-
AM406_RS00135	21822..22346	+	525	Protein_20	hypothetical protein	-
AM406_RS28825	22363..22846	+	484	Protein_21	hypothetical protein	-
AM406_RS28830	22844..23143	+	300	Protein_22	conjugal transfer protein TraH	-
AM406_RS00145	23225..24580	+	1356	WP_004187017.1	ISNCY family transposase	-
AM406_RS00150	24596..24880	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM406_RS00155	24870..25118	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
AM406_RS00160	25408..25832	-	425	Protein_26	IS1 family transposase	-
AM406_RS00165	26020..26136	-	117	Protein_27	transposase domain-containing protein	-
AM406_RS27810	26081..26344	-	264	WP_004187027.1	transposase	-
AM406_RS27815	26359..26622	+	264	WP_004118208.1	hypothetical protein	-
AM406_RS00175	26866..27147	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
AM406_RS00180	27182..27751	+	570	WP_004152117.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..153522	153522
	-	flank	IS/Tn	-	-	25372..25638	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T111233 WP_004187019.1 NZ_CP032173:c24880-24596 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T111233 NZ_CP032173:c24880-24596 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT111233 WP_004187025.1 NZ_CP032173:c25118-24870 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT111233 NZ_CP032173:c25118-24870 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4