Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9571..10124 Replicon plasmid unnamed5
Accession NZ_CP032172
Organism Klebsiella pneumoniae strain AR_0076

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7X5QH88
Locus tag AM455_RS28655 Protein ID WP_029403621.1
Coordinates 9810..10124 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A7X5QHA0
Locus tag AM455_RS28650 Protein ID WP_029403619.1
Coordinates 9571..9807 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM455_RS28625 5228..5872 + 645 WP_029403613.1 type IV secretion system protein -
AM455_RS28630 5884..6798 + 915 WP_029403614.1 P-type conjugative transfer protein TrbG -
AM455_RS28635 6799..7248 + 450 WP_110224566.1 hypothetical protein -
AM455_RS28640 7241..8455 + 1215 WP_050485028.1 hypothetical protein -
AM455_RS28645 8478..8966 + 489 WP_029403617.1 single-stranded DNA-binding protein -
AM455_RS30020 9056..9403 + 348 WP_029403618.1 hypothetical protein -
AM455_RS28650 9571..9807 + 237 WP_029403619.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM455_RS28655 9810..10124 + 315 WP_029403621.1 CcdB family protein Toxin
AM455_RS28665 10455..11381 + 927 WP_077759173.1 replication associated protein RepA1 -
AM455_RS28670 12179..12373 - 195 WP_029403586.1 hypothetical protein -
AM455_RS28675 12625..12936 - 312 WP_050485029.1 hypothetical protein -
AM455_RS28680 13089..13367 - 279 WP_029403588.1 hypothetical protein -
AM455_RS28685 13444..14106 - 663 WP_036944392.1 plasmid stability/partitioning protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..25871 25871


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11602.53 Da        Isoelectric Point: 5.7681

>T111232 WP_029403621.1 NZ_CP032172:9810-10124 [Klebsiella pneumoniae]
MQFTVYSNTGKSAVYPLLIDVTSDIIGQLNRRIVIPLLPVGMYPNGMRPERLTPLIRLVDDNEYVVMTHEMASIPARSLG
AAFCDASSYRKQIKDAIDFLLDGI

Download         Length: 315 bp

>T111232 NZ_CP032172:9810-10124 [Klebsiella pneumoniae]
ATGCAATTCACTGTATATAGTAATACAGGGAAAAGTGCCGTTTATCCCCTGCTGATTGATGTAACAAGCGATATTATCGG
CCAGTTAAACCGGAGGATTGTTATCCCCCTGTTACCTGTTGGAATGTACCCTAATGGTATGCGACCGGAGCGCCTGACTC
CGCTTATCAGGCTGGTTGATGATAATGAGTATGTAGTCATGACTCATGAAATGGCGAGTATTCCTGCTCGTTCTTTGGGG
GCTGCGTTCTGTGATGCTTCTTCTTATCGTAAGCAGATCAAGGATGCTATTGATTTTCTTCTCGATGGTATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9020.12 Da        Isoelectric Point: 5.6870

>AT111232 WP_029403619.1 NZ_CP032172:9571-9807 [Klebsiella pneumoniae]
MAVTQRPMQTVTMTVKRTLLTRAREAGINLSATLTAALDAELRQYEAKRWQEENSEALEALNRFHDEHGCFSDEYRTF

Download         Length: 237 bp

>AT111232 NZ_CP032172:9571-9807 [Klebsiella pneumoniae]
ATGGCTGTAACACAACGACCAATGCAAACCGTAACCATGACGGTAAAACGAACCTTGCTCACCCGTGCGCGTGAGGCTGG
AATTAATCTGAGCGCCACCCTGACCGCTGCCCTCGATGCTGAATTACGTCAGTACGAAGCTAAAAGGTGGCAAGAGGAAA
ACAGCGAAGCACTGGAAGCCCTCAATCGCTTCCACGACGAACACGGCTGTTTCAGTGATGAATACAGGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X5QH88

Antitoxin

Source ID Structure
AlphaFold DB A0A7X5QHA0

References