Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6481..7006 Replicon plasmid unnamed2
Accession NZ_CP032169
Organism Klebsiella pneumoniae strain AR_0076

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag AM455_RS27090 Protein ID WP_009309918.1
Coordinates 6701..7006 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag AM455_RS27085 Protein ID WP_006788213.1
Coordinates 6481..6699 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM455_RS27035 2171..2875 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM455_RS27045 3051..3812 + 762 Protein_4 IS5 family transposase -
AM455_RS27050 3908..4279 - 372 Protein_5 calmodulin-binding protein -
AM455_RS27055 4344..5048 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM455_RS27065 5238..5654 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
AM455_RS27070 5651..5881 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AM455_RS27085 6481..6699 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM455_RS27090 6701..7006 + 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
AM455_RS29915 7235..7375 + 141 WP_162898808.1 hypothetical protein -
AM455_RS27100 7425..7760 + 336 WP_009309920.1 hypothetical protein -
AM455_RS27105 7794..8810 + 1017 WP_009309921.1 hypothetical protein -
AM455_RS27110 9008..9787 + 780 WP_023287113.1 site-specific integrase -
AM455_RS27115 9845..10102 - 258 WP_009310077.1 hypothetical protein -
AM455_RS29925 10231..10344 - 114 WP_014343462.1 hypothetical protein -
AM455_RS27120 10876..11844 - 969 WP_000654811.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..171589 171589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T111229 WP_009309918.1 NZ_CP032169:6701-7006 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T111229 NZ_CP032169:6701-7006 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT111229 WP_006788213.1 NZ_CP032169:6481-6699 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT111229 NZ_CP032169:6481-6699 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References