Detailed information of TA system TA111228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5238..5881
• Replicon: plasmid unnamed2
• Accession: NZ_CP032169
• Organism: Klebsiella pneumoniae strain AR_0076

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A3Q8D6L7
• Locus tag: AM455_RS27065
• Protein ID: WP_020804312.1
• Coordinates: 5238..5654 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: AM455_RS27070
• Protein ID: WP_001261276.1
• Coordinates: 5651..5881 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM455_RS27030	838..1842	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
AM455_RS27035	2171..2875	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
AM455_RS27045	3051..3812	+	762	Protein_4	IS5 family transposase	-
AM455_RS27050	3908..4279	-	372	Protein_5	calmodulin-binding protein	-
AM455_RS27055	4344..5048	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
AM455_RS27065	5238..5654	-	417	WP_020804312.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM455_RS27070	5651..5881	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM455_RS27085	6481..6699	+	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
AM455_RS27090	6701..7006	+	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	-
AM455_RS29915	7235..7375	+	141	WP_162898808.1	hypothetical protein	-
AM455_RS27100	7425..7760	+	336	WP_009309920.1	hypothetical protein	-
AM455_RS27105	7794..8810	+	1017	WP_009309921.1	hypothetical protein	-
AM455_RS27110	9008..9787	+	780	WP_023287113.1	site-specific integrase	-
AM455_RS27115	9845..10102	-	258	WP_009310077.1	hypothetical protein	-
AM455_RS29925	10231..10344	-	114	WP_014343462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..171589	171589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15154.59 Da
• Isoelectric Point: 7.8637

>T111228 WP_020804312.1 NZ_CP032169:c5654-5238 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T111228 NZ_CP032169:c5654-5238 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT111228 WP_001261276.1 NZ_CP032169:c5881-5651 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT111228 NZ_CP032169:c5881-5651 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A3Q8D6L7

Antitoxin

• AlphaFold DB: A0A387K023