Detailed information of TA system TA111225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4672786..4673411
• Replicon: chromosome
• Accession: NZ_CP032167
• Organism: Klebsiella pneumoniae strain AR_0076

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM455_RS23695
• Protein ID: WP_021312635.1
• Coordinates: 4672786..4673169 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: AM455_RS23700
• Protein ID: WP_004150355.1
• Coordinates: 4673169..4673411 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM455_RS23680	4670152..4671054	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
AM455_RS23685	4671051..4671686	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
AM455_RS23690	4671683..4672612	+	930	WP_004146237.1	formate dehydrogenase accessory protein FdhE	-
AM455_RS23695	4672786..4673169	-	384	WP_021312635.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM455_RS23700	4673169..4673411	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM455_RS23705	4673616..4674533	+	918	WP_032412655.1	alpha/beta hydrolase	-
AM455_RS23710	4674548..4675489	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
AM455_RS23715	4675534..4675971	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM455_RS23720	4675968..4676828	-	861	WP_004146232.1	virulence factor BrkB family protein	-
AM455_RS23725	4676822..4677421	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14349.56 Da
• Isoelectric Point: 7.3178

>T111225 WP_021312635.1 NZ_CP032167:c4673169-4672786 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVATPYEIHPE

Nucleotide

• Length: 384 bp

>T111225 NZ_CP032167:c4673169-4672786 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGCCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT111225 WP_004150355.1 NZ_CP032167:c4673411-4673169 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT111225 NZ_CP032167:c4673411-4673169 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9