Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3509731..3510350 Replicon chromosome
Accession NZ_CP032167
Organism Klebsiella pneumoniae strain AR_0076

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM455_RS17935 Protein ID WP_002892050.1
Coordinates 3510132..3510350 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AM455_RS17930 Protein ID WP_002892066.1
Coordinates 3509731..3510105 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM455_RS17920 3504883..3506076 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM455_RS17925 3506099..3509245 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AM455_RS17930 3509731..3510105 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AM455_RS17935 3510132..3510350 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM455_RS17940 3510513..3511079 + 567 WP_119177337.1 maltose O-acetyltransferase -
AM455_RS29580 3511051..3511191 - 141 WP_004147370.1 hypothetical protein -
AM455_RS17945 3511212..3511682 + 471 WP_002892026.1 YlaC family protein -
AM455_RS17950 3511657..3513108 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
AM455_RS17955 3513209..3513907 + 699 WP_069197062.1 GNAT family N-acetyltransferase -
AM455_RS17960 3513904..3514044 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AM455_RS17965 3514044..3514307 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111221 WP_002892050.1 NZ_CP032167:3510132-3510350 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T111221 NZ_CP032167:3510132-3510350 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT111221 WP_002892066.1 NZ_CP032167:3509731-3510105 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT111221 NZ_CP032167:3509731-3510105 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References