Detailed information of TA system TA111210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 82264..82907
• Replicon: plasmid unnamed3
• Accession: NZ_CP032166
• Organism: Klebsiella pneumoniae strain XJ-K1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: D3H40_RS30015
• Protein ID: WP_001044770.1
• Coordinates: 82264..82680 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: D3H40_RS30020
• Protein ID: WP_001261282.1
• Coordinates: 82677..82907 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3H40_RS29980	77502..77939	+	438	WP_113248398.1	hypothetical protein	-
D3H40_RS29985	77966..78148	-	183	WP_102765728.1	hypothetical protein	-
D3H40_RS29990	78167..78367	-	201	Protein_114	hypothetical protein	-
D3H40_RS29995	78372..78653	-	282	Protein_115	restriction endonuclease	-
D3H40_RS30000	78668..79365	-	698	Protein_116	IS1 family transposase	-
D3H40_RS30005	79621..80643	-	1023	WP_000361404.1	helicase UvrD	-
D3H40_RS30010	80628..82190	-	1563	WP_004206609.1	AAA family ATPase	-
D3H40_RS30015	82264..82680	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
D3H40_RS30020	82677..82907	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D3H40_RS30025	82864..83325	+	462	WP_014343465.1	hypothetical protein	-
D3H40_RS30030	83486..84430	+	945	WP_011977810.1	hypothetical protein	-
D3H40_RS30035	84467..84859	+	393	WP_011977811.1	hypothetical protein	-
D3H40_RS30040	84917..85438	+	522	WP_013214008.1	hypothetical protein	-
D3H40_RS30045	85484..85687	+	204	WP_011977813.1	hypothetical protein	-
D3H40_RS30050	85717..86721	+	1005	WP_011977814.1	hypothetical protein	-
D3H40_RS30055	86905..87684	+	780	WP_013214009.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / blaSHV-12 / blaKPC-2	-	1..99048	99048

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T111210 WP_001044770.1 NZ_CP032166:c82680-82264 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T111210 NZ_CP032166:c82680-82264 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT111210 WP_001261282.1 NZ_CP032166:c82907-82677 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT111210 NZ_CP032166:c82907-82677 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3