Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 109452..109987 Replicon plasmid unnamed2
Accession NZ_CP032165
Organism Klebsiella pneumoniae strain XJ-K1

Toxin (Protein)


Gene name relE Uniprot ID A0A0M1UJC2
Locus tag D3H40_RS29130 Protein ID WP_001270422.1
Coordinates 109452..109739 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag D3H40_RS29135 Protein ID WP_001132895.1
Coordinates 109736..109987 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3H40_RS29100 104590..105759 - 1170 WP_001072358.1 hypothetical protein -
D3H40_RS29105 106126..106314 + 189 WP_001332784.1 hypothetical protein -
D3H40_RS29110 106435..107175 + 741 WP_001066947.1 tyrosine-type recombinase/integrase -
D3H40_RS29115 107418..108395 - 978 WP_001309253.1 RepB family plasmid replication initiator protein -
D3H40_RS29125 109039..109450 - 412 Protein_129 DDE-type integrase/transposase/recombinase -
D3H40_RS29130 109452..109739 - 288 WP_001270422.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D3H40_RS29135 109736..109987 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D3H40_RS29145 110745..111950 + 1206 WP_000817635.1 AAA family ATPase -
D3H40_RS29150 111947..112909 + 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
D3H40_RS29155 113143..113919 + 777 Protein_134 hypothetical protein -
D3H40_RS29165 114049..114549 - 501 WP_072164940.1 DUF4113 domain-containing protein -
D3H40_RS29175 114659..114970 + 312 WP_001309256.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / mph(A) senB 1..130628 130628
- flank IS/Tn - - 113566..113706 140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11262.30 Da        Isoelectric Point: 10.4703

>T111204 WP_001270422.1 NZ_CP032165:c109739-109452 [Klebsiella pneumoniae]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T111204 NZ_CP032165:c109739-109452 [Klebsiella pneumoniae]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT111204 WP_001132895.1 NZ_CP032165:c109987-109736 [Klebsiella pneumoniae]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT111204 NZ_CP032165:c109987-109736 [Klebsiella pneumoniae]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M1UJC2

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References