Detailed information of TA system TA111198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 13453..14123
• Replicon: plasmid unnamed1
• Accession: NZ_CP032164
• Organism: Klebsiella pneumoniae strain XJ-K1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: D3H40_RS27310
• Protein ID: WP_004213072.1
• Coordinates: 13680..14123 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: D3H40_RS27305
• Protein ID: WP_004213073.1
• Coordinates: 13453..13683 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3H40_RS27275	8638..9417	-	780	WP_004213560.1	site-specific integrase	-
D3H40_RS27280	9414..10235	-	822	WP_004213562.1	hypothetical protein	-
D3H40_RS27290	11207..11413	+	207	WP_004213077.1	hypothetical protein	-
D3H40_RS27295	11403..11696	-	294	WP_004213076.1	hypothetical protein	-
D3H40_RS27300	11712..12845	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
D3H40_RS27305	13453..13683	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D3H40_RS27310	13680..14123	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
D3H40_RS27315	14137..14553	-	417	Protein_17	restriction endonuclease	-
D3H40_RS27320	14557..14850	-	294	Protein_18	transposase	-
D3H40_RS30685	15267..15902	+	636	Protein_19	response regulator	-
D3H40_RS27340	16417..16823	-	407	Protein_20	GAF domain-containing protein	-
D3H40_RS27350	16914..17834	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
D3H40_RS27355	17883..18374	-	492	WP_011251327.1	DM13 domain-containing protein	-
D3H40_RS27360	18437..18712	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA	1..207409	207409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T111198 WP_004213072.1 NZ_CP032164:13680-14123 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T111198 NZ_CP032164:13680-14123 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT111198 WP_004213073.1 NZ_CP032164:13453-13683 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT111198 NZ_CP032164:13453-13683 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620