Detailed information of TA system TA111174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 847594..848123
• Replicon: chromosome
• Accession: NZ_CP032159
• Organism: Staphylococcus warneri strain 22.1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D3P10_RS04210
• Protein ID: WP_058709711.1
• Coordinates: 847761..848123 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2T4PXF4
• Locus tag: D3P10_RS04205
• Protein ID: WP_002451626.1
• Coordinates: 847594..847764 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3P10_RS04180	843419..843892	+	474	WP_119176350.1	PH domain-containing protein	-
D3P10_RS04185	843885..845411	+	1527	WP_058709716.1	PH domain-containing protein	-
D3P10_RS04190	845398..845901	+	504	WP_015364833.1	PH domain-containing protein	-
D3P10_RS04195	845928..846302	+	375	WP_058709709.1	holo-ACP synthase	-
D3P10_RS04200	846354..847502	+	1149	WP_058709710.1	alanine racemase	-
D3P10_RS04205	847594..847764	+	171	WP_002451626.1	hypothetical protein	Antitoxin
D3P10_RS04210	847761..848123	+	363	WP_058709711.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D3P10_RS04220	848446..849447	+	1002	WP_058709712.1	PP2C family protein-serine/threonine phosphatase	-
D3P10_RS04225	849541..849867	+	327	WP_002466754.1	anti-sigma factor antagonist	-
D3P10_RS04230	849869..850348	+	480	WP_002466768.1	anti-sigma B factor RsbW	-
D3P10_RS04235	850323..851093	+	771	WP_058709713.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.72 Da
• Isoelectric Point: 9.9784

>T111174 WP_058709711.1 NZ_CP032159:847761-848123 [Staphylococcus warneri]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSDNKMKEVDYALDISLGLHNFHHSKT

Nucleotide

• Length: 363 bp

>T111174 NZ_CP032159:847761-848123 [Staphylococcus warneri]
ATGATTAGAAGAGGAGATGTTTATCTAGCAGATTTATCACCTGTTCAAGGGTCAGAACAAGGGGGAGTAAGACCCGTCGT
CATTATCCAAAATGACACAGGTAATAAATATAGTCCTACTGTTATTGTTGCCGCAATCACTGGAAGGATTAATAAAGCTA
AGATACCTACACATGTTGAAATTGAAAAGAAAAAGTACAAACTTGATAAGGACTCAGTTATTTTATTAGAACAAATTCGT
ACTTTAGATAAAAATCGTTTAAAAGAAAAACTCACATATTTGTCTGATAACAAAATGAAAGAAGTAGATTACGCCCTAGA
CATCAGTCTAGGTTTACATAATTTCCATCATTCTAAGACATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6300.94 Da
• Isoelectric Point: 3.8309

>AT111174 WP_002451626.1 NZ_CP032159:847594-847764 [Staphylococcus warneri]
MLSFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNETYLTSNAKNE

Nucleotide

• Length: 171 bp

>AT111174 NZ_CP032159:847594-847764 [Staphylococcus warneri]
ATGTTATCTTTTAATCAAAATAGAAGTTACAGTTTAGAGCAATCTTTAAAAGAAGGTTATGCACAAATGGCTGATTTAAA
TCTCTCCCTAGCAACCGAAGCTTTTCCTATTGAATGTGAAGCGTGCGATTGCAATGAAACATATTTAACTTCTAACGCTA
AGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4PXF4