Detailed information of TA system TA111170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5054678..5055334
• Replicon: chromosome
• Accession: NZ_CP032155
• Organism: Mycobacterium sp. ELW1

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: D3H54_RS24105
• Protein ID: WP_149381853.1
• Coordinates: 5054678..5055124 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: D3H54_RS24110
• Protein ID: WP_149381855.1
• Coordinates: 5055128..5055334 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3H54_RS24080	5051406..5052290	+	885	WP_149381845.1	hypothetical protein	-
D3H54_RS24085	5052287..5053174	-	888	WP_149381847.1	amidohydrolase family protein	-
D3H54_RS24090	5053265..5053924	+	660	WP_149381849.1	response regulator transcription factor	-
D3H54_RS24095	5053926..5054153	-	228	WP_036341208.1	hypothetical protein	-
D3H54_RS24100	5054294..5054671	+	378	WP_149381851.1	hypothetical protein	-
D3H54_RS24105	5054678..5055124	-	447	WP_149381853.1	SRPBCC family protein	Toxin
D3H54_RS24110	5055128..5055334	-	207	WP_149381855.1	antitoxin	Antitoxin
D3H54_RS24115	5055338..5057740	-	2403	WP_149381857.1	HAD-IC family P-type ATPase	-
D3H54_RS24120	5057769..5059355	-	1587	WP_149381859.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-144)

Antitoxin

• Antitoxin Rv0909/MT0933 (9-55)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 16069.67 Da
• Isoelectric Point: 9.6249

>T111170 WP_149381853.1 NZ_CP032155:c5055124-5054678 [Mycobacterium sp. ELW1]
MAKLSVSVDVPLPPEQAWQHASDLSRYKDWLTIHRVWRSKLPETLEKGTVVESIVEVKGMPNRVKWEIVHFKAPEAMTLN
GAGVGGVKVKLIGKVRPNDSDANTSVVTMDVHLGGAALFGPIGMVVAGALKGDIKESLNRFVEVFSPS

Nucleotide

• Length: 447 bp

>T111170 NZ_CP032155:c5055124-5054678 [Mycobacterium sp. ELW1]
ATGGCGAAACTCTCTGTATCCGTTGACGTTCCATTACCGCCCGAGCAGGCGTGGCAGCACGCCTCCGACCTCTCGCGCTA
CAAGGACTGGCTGACCATCCACCGGGTGTGGCGCTCCAAGCTGCCCGAGACCCTCGAAAAGGGCACCGTCGTGGAGTCCA
TCGTCGAGGTCAAGGGCATGCCCAACCGGGTGAAGTGGGAGATCGTCCACTTCAAGGCGCCGGAAGCGATGACACTCAAC
GGCGCCGGTGTGGGTGGGGTCAAGGTCAAGCTGATCGGCAAGGTGCGGCCGAATGACTCCGACGCCAACACCTCGGTGGT
GACCATGGACGTCCACCTGGGCGGCGCGGCACTGTTCGGGCCGATCGGCATGGTCGTGGCCGGCGCGCTGAAGGGCGACA
TCAAGGAGTCGCTGAACCGCTTCGTCGAGGTCTTCAGTCCGTCCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7318.06 Da
• Isoelectric Point: 4.7265

>AT111170 WP_149381855.1 NZ_CP032155:c5055334-5055128 [Mycobacterium sp. ELW1]
MGFLDKALGKTKEVLGENADKVGQAIDKAGDIIDQKTQGKYSSTVDKVQDAAKNYVESNRTDKGDEPS

Nucleotide

• Length: 207 bp

>AT111170 NZ_CP032155:c5055334-5055128 [Mycobacterium sp. ELW1]
ATGGGATTCCTCGACAAAGCCCTCGGCAAGACCAAGGAAGTCTTGGGTGAGAATGCCGACAAGGTGGGCCAGGCCATCGA
CAAGGCCGGCGACATCATCGACCAGAAGACGCAGGGCAAGTACTCCAGCACCGTCGACAAGGTGCAGGATGCCGCCAAAA
ACTACGTCGAGTCCAACCGCACCGACAAGGGCGACGAACCAAGCTGA