Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 468737..469374 Replicon chromosome
Accession NZ_CP032146
Organism Bacillus amyloliquefaciens strain YP6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D2M30_RS02425 Protein ID WP_003156187.1
Coordinates 469024..469374 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag D2M30_RS02420 Protein ID WP_003156188.1
Coordinates 468737..469018 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2M30_RS02395 465102..465701 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
D2M30_RS02400 465794..466159 + 366 WP_125122079.1 holo-ACP synthase -
D2M30_RS02405 466566..466631 + 66 Protein_430 hypothetical protein -
D2M30_RS02415 467449..468618 + 1170 WP_125122081.1 alanine racemase -
D2M30_RS02420 468737..469018 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D2M30_RS02425 469024..469374 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D2M30_RS02430 469495..470316 + 822 WP_045508380.1 STAS domain-containing protein -
D2M30_RS02435 470321..470686 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
D2M30_RS02440 470689..471090 + 402 WP_013351097.1 anti-sigma regulatory factor -
D2M30_RS02445 471102..472109 + 1008 WP_065521858.1 PP2C family protein-serine/threonine phosphatase -
D2M30_RS02450 472173..472502 + 330 WP_014469786.1 anti-sigma factor antagonist -
D2M30_RS02455 472499..472981 + 483 WP_003156173.1 anti-sigma B factor RsbW -
D2M30_RS02460 472947..473735 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
D2M30_RS02465 473735..474337 + 603 WP_045508359.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T111165 WP_003156187.1 NZ_CP032146:469024-469374 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T111165 NZ_CP032146:469024-469374 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT111165 WP_003156188.1 NZ_CP032146:468737-469018 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT111165 NZ_CP032146:468737-469018 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References