Detailed information of TA system TA111163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 5092304..5093124
• Replicon: chromosome
• Accession: NZ_CP032145
• Organism: Escherichia coli strain 15.TR.026_OXA

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A0V9MKL8
• Locus tag: D3F09_RS24915
• Protein ID: WP_001550554.1
• Coordinates: 5092867..5093124 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: D3F09_RS24910
• Protein ID: WP_001519303.1
• Coordinates: 5092304..5092855 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3F09_RS24885	5087999..5089312	-	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-
D3F09_RS24890	5089324..5089602	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
D3F09_RS24895	5089599..5090720	-	1122	WP_000010833.1	M42 family metallopeptidase	-
D3F09_RS26075	5090965..5091081	-	117	Protein_4754	VOC family protein	-
D3F09_RS24900	5091119..5091379	-	261	WP_000084876.1	hypothetical protein	-
D3F09_RS24905	5091506..5092054	-	549	WP_001519301.1	hypothetical protein	-
D3F09_RS26080	5092091..5092252	-	162	WP_001519302.1	hypothetical protein	-
D3F09_RS24910	5092304..5092855	-	552	WP_001519303.1	N-acetyltransferase	Antitoxin
D3F09_RS24915	5092867..5093124	-	258	WP_001550554.1	hypothetical protein	Toxin
D3F09_RS24920	5093501..5093800	-	300	WP_001519304.1	GNAT family N-acetyltransferase	-
D3F09_RS24925	5093863..5095104	+	1242	Protein_4761	DNA helicase	-
D3F09_RS24930	5095687..5096667	-	981	WP_001519307.1	transposase	-
D3F09_RS24935	5096732..5097838	-	1107	WP_001519308.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	5092304..5104109	11805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9744.33 Da
• Isoelectric Point: 11.0090

>T111163 WP_001550554.1 NZ_CP032145:c5093124-5092867 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKPVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T111163 NZ_CP032145:c5093124-5092867 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGCCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT111163 WP_001519303.1 NZ_CP032145:c5092855-5092304 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT111163 NZ_CP032145:c5092855-5092304 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9MKL8