Detailed information of TA system TA111160

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4806359..4807193
• Replicon: chromosome
• Accession: NZ_CP032145
• Organism: Escherichia coli strain 15.TR.026_OXA

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: D3F09_RS23360
• Protein ID: WP_000854820.1
• Coordinates: 4806359..4806736 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: D3F09_RS23365
• Protein ID: WP_001285610.1
• Coordinates: 4806825..4807193 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3F09_RS23340	4802922..4804460	-	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-
D3F09_RS23345	4805295..4805855	-	561	Protein_4466	DUF4942 domain-containing protein	-
D3F09_RS23350	4805940..4806137	-	198	WP_085949158.1	DUF957 domain-containing protein	-
D3F09_RS23355	4806165..4806362	-	198	Protein_4468	hypothetical protein	-
D3F09_RS23360	4806359..4806736	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
D3F09_RS23365	4806825..4807193	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D3F09_RS23370	4807273..4807494	-	222	WP_000692347.1	DUF987 domain-containing protein	-
D3F09_RS23375	4807581..4808057	-	477	WP_001186193.1	RadC family protein	-
D3F09_RS23380	4808072..4808551	-	480	WP_000706978.1	antirestriction protein	-
D3F09_RS23385	4808817..4809635	-	819	WP_001175155.1	DUF945 domain-containing protein	-
D3F09_RS23390	4809725..4809958	-	234	WP_000883181.1	DUF905 family protein	-
D3F09_RS23395	4809964..4810641	-	678	WP_060579144.1	hypothetical protein	-
D3F09_RS23400	4810792..4811472	-	681	WP_021515201.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4795433..4840748	45315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T111160 WP_000854820.1 NZ_CP032145:c4806736-4806359 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T111160 NZ_CP032145:c4806736-4806359 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT111160 WP_001285610.1 NZ_CP032145:c4807193-4806825 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT111160 NZ_CP032145:c4807193-4806825 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6