Detailed information of TA system TA111129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1215956..1216873
• Replicon: chromosome
• Accession: NZ_CP032144
• Organism: Bacillus velezensis strain BIM B-439D

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: D3C60_RS06435
• Protein ID: WP_032866111.1
• Coordinates: 1216127..1216873 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: D3C60_RS06430
• Protein ID: WP_003154807.1
• Coordinates: 1215956..1216126 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3C60_RS06395	1211189..1212811	+	1623	WP_032866113.1	pyocin knob domain-containing protein	-
D3C60_RS06400	1212824..1213195	+	372	WP_020955692.1	hypothetical protein	-
D3C60_RS06405	1213200..1213397	+	198	WP_020955693.1	XkdX family protein	-
D3C60_RS06410	1213454..1214215	+	762	WP_020955694.1	hypothetical protein	-
D3C60_RS06415	1214267..1214530	+	264	WP_003154815.1	hemolysin XhlA family protein	-
D3C60_RS06420	1214544..1214807	+	264	WP_003154813.1	phage holin	-
D3C60_RS06425	1214821..1215699	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
D3C60_RS06430	1215956..1216126	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
D3C60_RS06435	1216127..1216873	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
D3C60_RS06440	1216977..1217975	-	999	WP_020955696.1	inorganic phosphate transporter	-
D3C60_RS06445	1217988..1218605	-	618	WP_003154804.1	DUF47 domain-containing protein	-
D3C60_RS06450	1218891..1220207	-	1317	WP_007610842.1	amino acid permease	-
D3C60_RS06455	1220530..1221480	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1170978..1253069	82091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T111129 WP_032866111.1 NZ_CP032144:c1216873-1216127 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T111129 NZ_CP032144:c1216873-1216127 [Bacillus velezensis]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT111129 WP_003154807.1 NZ_CP032144:c1216126-1215956 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT111129 NZ_CP032144:c1216126-1215956 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14