Detailed information of TA system TA111128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 486394..487031
• Replicon: chromosome
• Accession: NZ_CP032144
• Organism: Bacillus velezensis strain BIM B-439D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: D3C60_RS02495
• Protein ID: WP_003156187.1
• Coordinates: 486681..487031 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: D3C60_RS02490
• Protein ID: WP_003156188.1
• Coordinates: 486394..486675 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3C60_RS02470	482759..483358	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
D3C60_RS02475	483451..483816	+	366	WP_007410229.1	holo-ACP synthase	-
D3C60_RS02480	483981..484988	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
D3C60_RS02485	485105..486274	+	1170	WP_032866888.1	alanine racemase	-
D3C60_RS02490	486394..486675	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
D3C60_RS02495	486681..487031	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D3C60_RS02500	487149..487970	+	822	WP_003156182.1	STAS domain-containing protein	-
D3C60_RS02505	487975..488340	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
D3C60_RS02510	488343..488744	+	402	WP_003156178.1	anti-sigma regulatory factor	-
D3C60_RS02515	488756..489763	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
D3C60_RS02520	489827..490156	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
D3C60_RS02525	490153..490635	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
D3C60_RS02530	490601..491389	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
D3C60_RS02535	491389..491991	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T111128 WP_003156187.1 NZ_CP032144:486681-487031 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T111128 NZ_CP032144:486681-487031 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT111128 WP_003156188.1 NZ_CP032144:486394-486675 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT111128 NZ_CP032144:486394-486675 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5