Detailed information of TA system TA111121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 20733..21321
• Replicon: plasmid pOXA58_010005
• Accession: NZ_CP032133
• Organism: Acinetobacter chinensis strain WCHAc010005

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9MLD7
• Locus tag: CDG60_RS00510
• Protein ID: WP_005175534.1
• Coordinates: 20733..21029 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDG60_RS00515
• Protein ID: WP_004969304.1
• Coordinates: 21031..21321 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG60_RS00480	16211..17509	+	1299	WP_020753602.1	Y-family DNA polymerase	-
CDG60_RS00485	17506..17700	-	195	WP_020753603.1	exodeoxyribonuclease VII small subunit	-
CDG60_RS00490	17693..18955	-	1263	WP_020753604.1	exodeoxyribonuclease VII large subunit	-
CDG60_RS00495	19056..19760	-	705	WP_020753605.1	IS6 family transposase	-
CDG60_RS00500	19823..20017	-	195	Protein_16	recombinase family protein	-
CDG60_RS00505	20281..20550	-	270	WP_039048204.1	hypothetical protein	-
CDG60_RS00510	20733..21029	+	297	WP_005175534.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG60_RS00515	21031..21321	+	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
CDG60_RS00525	21477..22802	+	1326	WP_079393426.1	ISNCY family transposase	-
CDG60_RS00530	22879..23193	-	315	WP_038350244.1	BrnA antitoxin family protein	-
CDG60_RS00535	23156..23467	-	312	WP_038350243.1	BrnT family toxin	-
CDG60_RS00545	23957..24982	+	1026	WP_010326927.1	IS30 family transposase	-
CDG60_RS00550	25083..25862	+	780	WP_000422636.1	aminoglycoside O-phosphotransferase APH(3')-VIa	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / blaOXA-58 / cmlB1 / aac(3)-IId	-	1..66715	66715
	-	inside	IScluster/Tn	aph(3')-VIa / blaOXA-58	-	9737..35757	26020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11568.25 Da
• Isoelectric Point: 5.8801

>T111121 WP_005175534.1 NZ_CP032133:20733-21029 [Acinetobacter chinensis]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T111121 NZ_CP032133:20733-21029 [Acinetobacter chinensis]
ATGATTGAAATTAAACGTCTGCCAGAGTTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATTCGCCTGAA
TCGTAGATTGGATAAGGTTCAACGTGGAAATTGGGGAGACATTAAGCCTCTTCAAGATGGCGTTTGGGAAATGAGAGAGT
TCTTCGGTGCTGGATGGAGAATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGTGGAGGAGATAAATCG
ACTCAACAACAAGATATCGATCGTGCAGTCAAATTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT111121 WP_004969304.1 NZ_CP032133:21031-21321 [Acinetobacter chinensis]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT111121 NZ_CP032133:21031-21321 [Acinetobacter chinensis]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCGCTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGAGAATGATCCAGCTGAATTAGCCCATGCACTTGGCGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGGATTGGACGTGAGGCACTCTACAAAGCCCTACGTCAGGATTCAGCACCACGTTTTGATACGATT
AATCGTGTTGTGAATGCTTTAGGCCTAAAACTCACAGTACAACATGCCTAA

Structures

Toxin

• AlphaFold DB: N9MLD7