111119

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4717813..4718421 Replicon chromosome
Accession NZ_CP032126
Organism Pseudomonas aeruginosa strain PAO1161

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag D3C65_RS22380 Protein ID WP_003114156.1
Coordinates 4717813..4718160 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag D3C65_RS22385 Protein ID WP_003114155.1
Coordinates 4718170..4718421 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3C65_RS22350 4713172..4713444 + 273 WP_010895521.1 cysteine-rich CWC family protein -
D3C65_RS22355 4713444..4714136 + 693 WP_003114159.1 16S rRNA pseudouridine(516) synthase -
D3C65_RS22360 4714272..4715315 + 1044 WP_003098363.1 L,D-transpeptidase -
D3C65_RS22365 4715395..4716132 + 738 WP_003114158.1 murein L,D-transpeptidase catalytic domain family protein -
D3C65_RS22370 4716584..4717486 + 903 WP_003114157.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
D3C65_RS22380 4717813..4718160 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D3C65_RS22385 4718170..4718421 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D3C65_RS22390 4718635..4719618 - 984 WP_003114154.1 tyrosine-type recombinase/integrase -
D3C65_RS22395 4719618..4720910 - 1293 WP_003115206.1 hypothetical protein -
D3C65_RS22400 4721140..4722414 - 1275 WP_010895520.1 zonular occludens toxin family protein -
D3C65_RS22405 4722418..4722774 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4708709..4739927 31218


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T111119 WP_003114156.1 NZ_CP032126:c4718160-4717813 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T111119 NZ_CP032126:c4718160-4717813 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT111119 WP_003114155.1 NZ_CP032126:c4718421-4718170 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT111119 NZ_CP032126:c4718421-4718170 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References