Detailed information of TA system TA111111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1710..2277
• Replicon: plasmid pALWEK1.6
• Accession: NZ_CP032108
• Organism: Acinetobacter lwoffii strain EK30A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9QF31
• Locus tag: ABEKA_RS01340
• Protein ID: WP_005098405.1
• Coordinates: 1710..1991 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9NWR9
• Locus tag: ABEKA_RS01345
• Protein ID: WP_005098404.1
• Coordinates: 1984..2277 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABEKA_RS01335 (ABWEK_E0001)	842..1651	-	810	WP_005098406.1	hypothetical protein	-
ABEKA_RS01340 (ABWEK_E0002)	1710..1991	-	282	WP_005098405.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ABEKA_RS01345 (ABWEK_E0003)	1984..2277	-	294	WP_005098404.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ABEKA_RS01350 (ABWEK_E0004)	2421..2921	-	501	WP_005098403.1	hypothetical protein	-
ABEKA_RS01355 (ABWEK_E0005)	2918..3847	-	930	WP_005249378.1	MobA/MobL family protein	-
ABEKA_RS01360 (ABWEK_E0006)	3847..4002	-	156	WP_005098410.1	hypothetical protein	-
ABEKA_RS01365 (ABWEK_E0007)	4279..4698	+	420	WP_005098409.1	conjugal transfer protein TraD	-
ABEKA_RS01370 (ABWEK_E0008)	4699..5307	-	609	WP_004282301.1	LysE family translocator	-
ABEKA_RS01375 (ABWEK_E0009)	5309..6874	-	1566	WP_005098407.1	tryptophan 7-halogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6886	6886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-90)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10693.41 Da
• Isoelectric Point: 6.8520

>T111111 WP_005098405.1 NZ_CP032108:c1991-1710 [Acinetobacter lwoffii]
MAKRKIIVTSSFKRDIKRRYLDLVTAEWAEVLDCLVANSPLPEKYLDHPLKGGAEFKNCRDCHVKPDLVLIYRLVGDDVL
ELHQLDSHSEIFG

Nucleotide

• Length: 282 bp

>T111111 NZ_CP032108:c1991-1710 [Acinetobacter lwoffii]
ATGGCTAAACGAAAAATAATCGTAACCAGTTCATTTAAAAGAGACATTAAAAGACGTTATTTAGATTTGGTTACGGCTGA
ATGGGCCGAGGTACTGGACTGTTTAGTTGCTAATAGTCCATTACCTGAAAAATATCTAGATCATCCATTAAAAGGTGGTG
CTGAGTTTAAAAATTGTCGAGATTGCCACGTTAAACCTGATTTAGTCTTGATTTATAGGCTCGTAGGGGATGATGTTTTA
GAATTACACCAGTTAGATAGCCATTCAGAAATTTTTGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10948.52 Da
• Isoelectric Point: 4.3170

>AT111111 WP_005098404.1 NZ_CP032108:c2277-1984 [Acinetobacter lwoffii]
MSAVNFNMRLEAELKEKVTPILEDYGLTMPQAFKLFLNQIVKTKTIPLSFDYARETALTPKAAAKLLQSLKEIENGEYTE
YETVEEAIQAMSEEANG

Nucleotide

• Length: 294 bp

>AT111111 NZ_CP032108:c2277-1984 [Acinetobacter lwoffii]
ATGTCCGCAGTCAATTTCAATATGCGTTTAGAGGCAGAACTGAAAGAAAAAGTTACTCCTATTTTGGAGGACTACGGTTT
AACCATGCCACAGGCATTTAAACTGTTCTTAAATCAAATTGTTAAAACGAAAACGATTCCATTGTCTTTTGATTATGCAA
GGGAAACAGCTTTAACCCCTAAAGCAGCAGCAAAGTTGCTTCAATCTCTTAAAGAAATTGAAAATGGGGAGTACACCGAG
TATGAAACAGTAGAAGAAGCCATCCAAGCAATGTCGGAAGAAGCTAATGGCTAA

Structures

Toxin

• AlphaFold DB: N9QF31

Antitoxin

• AlphaFold DB: N9NWR9