Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 164191..164779 Replicon plasmid pALWEK1.1
Accession NZ_CP032102
Organism Acinetobacter lwoffii strain EK30A

Toxin (Protein)


Gene name relE Uniprot ID N9MLD7
Locus tag ABEKA_RS00860 Protein ID WP_005175534.1
Coordinates 164483..164779 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2S4T219
Locus tag ABEKA_RS00855 Protein ID WP_060875721.1
Coordinates 164191..164481 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABEKA_RS00840 160700..161101 + 402 Protein_167 hypothetical protein -
ABEKA_RS00845 161192..162324 + 1133 WP_223287772.1 IS3 family transposase -
ABEKA_RS00850 (ABWEK_A0164) 162625..164079 + 1455 WP_019838442.1 SulP family inorganic anion transporter -
ABEKA_RS00855 (ABWEK_A0165) 164191..164481 - 291 WP_060875721.1 putative addiction module antidote protein Antitoxin
ABEKA_RS00860 (ABWEK_A0166) 164483..164779 - 297 WP_005175534.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ABEKA_RS00865 (ABWEK_A0168) 165124..165780 + 657 WP_159373176.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
ABEKA_RS00870 (ABWEK_A0169) 165785..166492 + 708 WP_159373184.1 hypothetical protein -
ABEKA_RS00875 (ABWEK_A0171) 166788..167315 - 528 WP_004811816.1 plasmid replication DNA-binding protein -
ABEKA_RS00880 (ABWEK_A0172) 167400..168323 - 924 WP_159373177.1 replication initiation protein RepM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..209982 209982


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-91)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-92)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11568.25 Da        Isoelectric Point: 5.8801

>T111109 WP_005175534.1 NZ_CP032102:c164779-164483 [Acinetobacter lwoffii]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Download         Length: 297 bp

>T111109 NZ_CP032102:c164779-164483 [Acinetobacter lwoffii]
ATGATTGAAATTAAACGTCTGCCAGAATTTGATGAATGGTTAGATGGCATTAAAGACAATATGACCCGTATTCGTCTGAA
TCGTAGATTAGATAAGGTTCAACGTGGAAATTGGGGAGACATTAAGCCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTGCTGGATGGAGAATGTATTACATCCAGCATGGTGACGTAGTGATTGTAATGCTTGGTGGAGGAGATAAATCG
ACTCAACAACAAGACATCGATCGTGCAGTCAAATTATCGAAAACATTGGAGGATTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10519.17 Da        Isoelectric Point: 5.6088

>AT111109 WP_060875721.1 NZ_CP032102:c164481-164191 [Acinetobacter lwoffii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEDNDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRHDSAPRFDTI
NRVVNALGLKLTVQHA

Download         Length: 291 bp

>AT111109 NZ_CP032102:c164481-164191 [Acinetobacter lwoffii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGATAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TTGCTAAAGAAGCAGGGATTGGGCGTGAGGCACTCTACAAAGCCCTACGTCATGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCGTTAGGCTTAAAACTCACTGTACAACACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB N9MLD7

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4T219

References