Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 27414..27936 Replicon plasmid pL25922_1
Accession NZ_CP032087
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag D1792_RS25440 Protein ID WP_000220560.1
Coordinates 27414..27695 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag D1792_RS25445 Protein ID WP_000121743.1
Coordinates 27685..27936 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS25390 23519..23983 - 465 WP_001443073.1 hypothetical protein -
D1792_RS25395 24240..24626 - 387 WP_000273881.1 hypothetical protein -
D1792_RS25400 24702..25055 - 354 Protein_30 H-NS histone family protein -
D1792_RS25405 25286..25558 - 273 WP_000160399.1 hypothetical protein -
D1792_RS25410 25571..25720 - 150 WP_000003880.1 hypothetical protein -
D1792_RS25415 25790..25985 - 196 Protein_33 hypothetical protein -
D1792_RS25420 26028..26357 - 330 WP_000866648.1 hypothetical protein -
D1792_RS25425 26450..26665 - 216 WP_001180116.1 hypothetical protein -
D1792_RS25430 26655..26900 - 246 WP_000356548.1 hypothetical protein -
D1792_RS25435 26945..27268 - 324 WP_001181903.1 hypothetical protein -
D1792_RS25440 27414..27695 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D1792_RS25445 27685..27936 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
D1792_RS25450 29158..29997 + 840 WP_001546350.1 replication initiation protein -
D1792_RS25455 30018..30855 + 838 Protein_41 replication initiation protein -
D1792_RS25460 30859..31269 + 411 Protein_42 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37009 37009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T111093 WP_000220560.1 NZ_CP032087:c27695-27414 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T111093 NZ_CP032087:c27695-27414 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATTA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT111093 WP_000121743.1 NZ_CP032087:c27936-27685 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT111093 NZ_CP032087:c27936-27685 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References