Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4554739..4555357 Replicon chromosome
Accession NZ_CP032085
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D1792_RS21785 Protein ID WP_001291435.1
Coordinates 4554739..4554957 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag D1792_RS21790 Protein ID WP_000344800.1
Coordinates 4554983..4555357 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS21750 4550027..4550599 + 573 WP_000779831.1 YbaY family lipoprotein -
D1792_RS21755 4550630..4550941 - 312 WP_000409908.1 MGMT family protein -
D1792_RS21765 4551320..4551673 + 354 WP_000878140.1 DUF1428 domain-containing protein -
D1792_RS21770 4551715..4553265 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D1792_RS21775 4553429..4553899 - 471 WP_001304825.1 YlaC family protein -
D1792_RS21780 4554015..4554566 - 552 WP_000102539.1 maltose O-acetyltransferase -
D1792_RS21785 4554739..4554957 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
D1792_RS21790 4554983..4555357 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
D1792_RS21795 4555902..4559051 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
D1792_RS21800 4559074..4560267 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111089 WP_001291435.1 NZ_CP032085:c4554957-4554739 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T111089 NZ_CP032085:c4554957-4554739 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT111089 WP_000344800.1 NZ_CP032085:c4555357-4554983 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT111089 NZ_CP032085:c4555357-4554983 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References