Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4259721..4260519 Replicon chromosome
Accession NZ_CP032085
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag D1792_RS20425 Protein ID WP_000854730.1
Coordinates 4259721..4260098 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag D1792_RS20430 Protein ID WP_001285481.1
Coordinates 4260145..4260519 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS20400 4254977..4256381 + 1405 Protein_3983 IS66 family transposase -
D1792_RS20405 4256635..4257768 - 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
D1792_RS20410 4257829..4258017 + 189 Protein_3985 IS66 family transposase -
D1792_RS20415 4258165..4259153 + 989 Protein_3986 IS630 family transposase -
D1792_RS20420 4259232..4259724 - 493 Protein_3987 hypothetical protein -
D1792_RS20425 4259721..4260098 - 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
D1792_RS20430 4260145..4260519 - 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
D1792_RS20435 4260569..4261213 - 645 WP_000086761.1 hypothetical protein -
D1792_RS20440 4261232..4261453 - 222 WP_000692315.1 DUF987 domain-containing protein -
D1792_RS20445 4261516..4261992 - 477 WP_001313574.1 RadC family protein -
D1792_RS20450 4262008..4262481 - 474 WP_001313575.1 antirestriction protein -
D1792_RS20455 4262575..4262820 - 246 WP_001164966.1 hypothetical protein -
D1792_RS20460 4262820..4263641 - 822 WP_001234359.1 DUF945 domain-containing protein -
D1792_RS20465 4263741..4263974 - 234 WP_001213776.1 DUF905 family protein -
D1792_RS20470 4264092..4264544 - 453 WP_000682723.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4250821..4307576 56755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T111088 WP_000854730.1 NZ_CP032085:c4260098-4259721 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T111088 NZ_CP032085:c4260098-4259721 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT111088 WP_001285481.1 NZ_CP032085:c4260519-4260145 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT111088 NZ_CP032085:c4260519-4260145 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References