Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4060823..4061373 Replicon chromosome
Accession NZ_CP032085
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag D1792_RS19495 Protein ID WP_001160966.1
Coordinates 4061059..4061373 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag D1792_RS19490 Protein ID WP_000125566.1
Coordinates 4060823..4061056 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS19465 4055896..4056183 + 288 WP_000203741.1 ferredoxin-like protein FixX -
D1792_RS19470 4056242..4057573 + 1332 WP_001183207.1 MFS transporter -
D1792_RS19475 4057681..4058211 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
D1792_RS19480 4058204..4060066 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
D1792_RS19485 4060258..4060737 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
D1792_RS19490 4060823..4061056 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
D1792_RS19495 4061059..4061373 + 315 WP_001160966.1 CcdB family protein Toxin
D1792_RS19500 4061370..4062218 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
D1792_RS19505 4062225..4062602 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
D1792_RS19510 4062605..4063426 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
D1792_RS19515 4063423..4064412 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
D1792_RS19520 4064412..4065698 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T111087 WP_001160966.1 NZ_CP032085:4061059-4061373 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T111087 NZ_CP032085:4061059-4061373 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT111087 WP_000125566.1 NZ_CP032085:4060823-4061056 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT111087 NZ_CP032085:4060823-4061056 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References