Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2163154..2163808 Replicon chromosome
Accession NZ_CP032085
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag D1792_RS10405 Protein ID WP_000244781.1
Coordinates 2163154..2163561 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D1792_RS10410 Protein ID WP_000354046.1
Coordinates 2163542..2163808 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS10385 2159111..2160844 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
D1792_RS10390 2160850..2161560 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D1792_RS10395 2161585..2162481 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D1792_RS10400 2162593..2163114 + 522 WP_001055874.1 flavodoxin FldB -
D1792_RS10405 2163154..2163561 - 408 WP_000244781.1 protein YgfX Toxin
D1792_RS10410 2163542..2163808 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D1792_RS10415 2164051..2165031 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
D1792_RS10420 2165108..2165767 - 660 WP_000250274.1 hemolysin III family protein -
D1792_RS10425 2165931..2166242 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
D1792_RS10430 2166287..2167720 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T111076 WP_000244781.1 NZ_CP032085:c2163561-2163154 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T111076 NZ_CP032085:c2163561-2163154 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT111076 WP_000354046.1 NZ_CP032085:c2163808-2163542 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT111076 NZ_CP032085:c2163808-2163542 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References