Detailed information of TA system TA111062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 162407..162878
• Replicon: chromosome
• Accession: NZ_CP032085
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: D1792_RS00845
• Protein ID: WP_001303511.1
• Coordinates: 162407..162685 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: D1792_RS00850
• Protein ID: WP_001302048.1
• Coordinates: 162687..162878 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1792_RS00810	157619..157888	-	270	WP_000003742.1	excisionase	-
D1792_RS00815	157950..160388	-	2439	WP_000102132.1	exonuclease	-
D1792_RS00820	160481..160669	-	189	WP_000199475.1	DUF1482 family protein	-
D1792_RS00825	160666..160854	-	189	WP_000449175.1	cell division inhibitor	-
D1792_RS00830	161358..161624	+	267	WP_012601410.1	hypothetical protein	-
D1792_RS00835	161613..161951	-	339	WP_000394541.1	hypothetical protein	-
D1792_RS00840	161963..162115	-	153	WP_000380313.1	DUF1391 family protein	-
D1792_RS00845	162407..162685	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1792_RS00850	162687..162878	-	192	WP_001302048.1	hypothetical protein	Antitoxin
D1792_RS00855	162899..163270	-	372	WP_001169687.1	hypothetical protein	-
D1792_RS00860	163368..163670	+	303	WP_001556930.1	transcriptional regulator	-
D1792_RS00865	163667..164092	+	426	WP_000693943.1	Rha family transcriptional regulator	-
D1792_RS00870	164115..165077	+	963	WP_000095673.1	helix-turn-helix domain-containing protein	-
D1792_RS00875	165084..165830	+	747	WP_000788950.1	ATP-binding protein	-
D1792_RS00880	165852..166622	+	771	WP_000450998.1	DUF1627 domain-containing protein	-
D1792_RS00885	166638..167060	+	423	Protein_174	DUF977 family protein	-
D1792_RS00890	167166..167378	+	213	WP_000072553.1	hypothetical protein	-
D1792_RS00895	167411..167629	+	219	WP_000209148.1	DUF4014 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	-	149364..299769	150405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T111062 WP_001303511.1 NZ_CP032085:c162685-162407 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T111062 NZ_CP032085:c162685-162407 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT111062 WP_001302048.1 NZ_CP032085:c162878-162687 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT111062 NZ_CP032085:c162878-162687 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0