Detailed information of TA system TA111057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 2291..2792
• Replicon: plasmid p.CT30-P3
• Accession: NZ_CP032081
• Organism: Escherichia coli strain CT30

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: D2195_RS25615
• Protein ID: WP_000702247.1
• Coordinates: 2291..2551 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: D2195_RS25620
• Protein ID: WP_001132407.1
• Coordinates: 2541..2792 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2195_RS25595 (D2195_27700)	433..762	+	330	WP_000855120.1	hypothetical protein	-
D2195_RS25600 (D2195_27710)	1344..1667	+	324	WP_000427403.1	hypothetical protein	-
D2195_RS25605 (D2195_27715)	1709..1918	+	210	WP_000866036.1	hypothetical protein	-
D2195_RS25610 (D2195_27720)	2015..2239	+	225	WP_000814381.1	hypothetical protein	-
D2195_RS25615 (D2195_27725)	2291..2551	+	261	WP_000702247.1	hypothetical protein	Toxin
D2195_RS25620 (D2195_27730)	2541..2792	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
D2195_RS25625 (D2195_27745)	3342..3620	+	279	WP_000545938.1	hypothetical protein	-
D2195_RS25630 (D2195_27750)	4040..4306	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
D2195_RS26465	4413..4856	-	444	WP_000068545.1	hypothetical protein	-
D2195_RS25640 (D2195_27760)	5153..5335	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
D2195_RS25645 (D2195_27765)	5360..5797	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
D2195_RS25650 (D2195_27770)	5928..6254	-	327	WP_063078684.1	hypothetical protein	-
D2195_RS25655 (D2195_27775)	6274..6981	-	708	WP_021503228.1	hypothetical protein	-
D2195_RS25660 (D2195_27780)	6983..7735	-	753	WP_063078685.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60733	60733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T111057 WP_000702247.1 NZ_CP032081:2291-2551 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T111057 NZ_CP032081:2291-2551 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT111057 WP_001132407.1 NZ_CP032081:2541-2792 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT111057 NZ_CP032081:2541-2792 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0