Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4464105..4464602 Replicon chromosome
Accession NZ_CP032078
Organism Escherichia coli strain CT30

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag D2195_RS21700 Protein ID WP_001173343.1
Coordinates 4464105..4464278 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag D2195_RS21705 Protein ID WP_001317561.1
Coordinates 4464306..4464602 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2195_RS21690 (4460775) 4460775..4462232 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
D2195_RS21695 (4462469) 4462469..4463986 + 1518 WP_000003805.1 lysine--tRNA ligase -
D2195_RS21700 (4464105) 4464105..4464278 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
D2195_RS21705 (4464306) 4464306..4464602 - 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
D2195_RS21710 (4464829) 4464829..4465101 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
D2195_RS21715 (4465113) 4465113..4465343 - 231 WP_000371717.1 4Fe-4S mono-cluster protein YjdI -
D2195_RS21720 (4465523) 4465523..4467154 + 1632 WP_212408102.1 sensor histidine kinase -
D2195_RS21725 (4467151) 4467151..4467870 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T111043 WP_001173343.1 NZ_CP032078:c4464278-4464105 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T111043 NZ_CP032078:c4464278-4464105 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT111043 WP_001317561.1 NZ_CP032078:c4464602-4464306 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT111043 NZ_CP032078:c4464602-4464306 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References