Detailed information of TA system TA111029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2963321..2963957
• Replicon: chromosome
• Accession: NZ_CP032078
• Organism: Escherichia coli strain CT30

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: D2195_RS14250
• Protein ID: WP_000813796.1
• Coordinates: 2963321..2963497 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D2195_RS14255
• Protein ID: WP_076838470.1
• Coordinates: 2963541..2963957 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2195_RS14230 (2958943)	2958943..2960154	-	1212	WP_071593090.1	BenE family transporter YdcO	-
D2195_RS14235 (2960207)	2960207..2960743	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
D2195_RS14240 (2960816)	2960816..2962777	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D2195_RS14245 (2962869)	2962869..2963099	-	231	WP_000494243.1	YncJ family protein	-
D2195_RS14250 (2963321)	2963321..2963497	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D2195_RS14255 (2963541)	2963541..2963957	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D2195_RS14260 (2964036)	2964036..2965442	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
D2195_RS14265 (2965687)	2965687..2966832	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
D2195_RS14270 (2966850)	2966850..2967863	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
D2195_RS14275 (2967864)	2967864..2968805	+	942	WP_001251307.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2946330..2963957	17627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T111029 WP_000813796.1 NZ_CP032078:2963321-2963497 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T111029 NZ_CP032078:2963321-2963497 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT111029 WP_076838470.1 NZ_CP032078:2963541-2963957 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT111029 NZ_CP032078:2963541-2963957 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3